Abstract: Using nitrogenous fertilizer is a basic way to increase agricultural output. Meanwhile,nitrogen removal by soil denitrification can dissipate the residual nitrogen that infiltrates into the soil,leading to protection groundwater from pollution. Factors influencing the measurement of nitrogen removal by soil denitrification are complicated and there is no relatively perfect methods for measurement wildly used at present. Based on discussion of essence and influence of denitrification in soil laye...